How DE-T1 Influences Human Ovarian Granulosa Cells and Hormone Receptors
Scientific research into SHAWKEA T1 has explored the biological activity of DE-T1, a key dandelion-derived component contained in SHAWKEA T1, and how it interacts with cells involved in ovarian function.
A study published in the Chinese Medical Journal investigated the effects of DE-T1 on human ovarian granulosa cells, focusing on cell proliferation, hormone-receptor expression and steroid-hormone production.
The research provides an important insight into the potential biological mechanisms behind DE-T1 and contributes to the growing scientific research surrounding SHAWKEA T1.
What Is DE-T1?
DE-T1 is a dandelion-derived component contained in SHAWKEA T1 and has been the subject of scientific research investigating its biological activity.
In this study, researchers examined DE-T1 directly under controlled laboratory conditions to understand how it interacts with human ovarian granulosa cells.
This allows researchers to investigate the cellular mechanisms associated with the DE-T1 component of SHAWKEA T1 in greater detail.
Why Are Granulosa Cells Important?
Granulosa cells are specialised cells that surround and support the developing egg inside an ovarian follicle.
They play an important role in the follicular environment and respond to reproductive hormones such as follicle-stimulating hormone (FSH) and luteinising hormone (LH). They are also involved in the production of hormones including oestrogen and progesterone.
For this reason, understanding granulosa-cell activity and hormone-receptor expression is an important area of ovarian research.
What Did the Researchers Study?
The study involved ovarian granulosa cells collected from 60 women undergoing IVF.
Participants were divided into two groups:
- Normal ovarian response (NOR)
- Poor ovarian response (POR)
The researchers isolated the granulosa cells and exposed them to different concentrations of DE-T1, the dandelion-derived component contained in SHAWKEA T1, under laboratory conditions.
They then examined several important biological markers:
FSHR — the receptor for follicle-stimulating hormone (FSH)
LHR — the receptor associated with luteinising hormone (LH)
IGF-1R — the insulin-like growth factor-1 receptor, involved in cellular growth and signalling
CYP19A1 (aromatase) — an enzyme involved in oestrogen production
The researchers also measured the production of oestradiol (E2) and progesterone.
DE-T1 and Granulosa-Cell Activity
One of the main findings was increased granulosa-cell viability with increasing concentrations of DE-T1 within the experimental conditions.
After 48 hours, several DE-T1 concentrations produced significantly greater proliferative activity than the untreated control.
In granulosa cells obtained from patients with poor ovarian response, the researchers also reported that DE-T1 delayed cellular ageing, loss of viability and declining proliferative ability compared with untreated cells.
These laboratory findings suggest that DE-T1 contained in SHAWKEA T1 can influence the activity and viability of human ovarian granulosa cells under laboratory conditions.
Changes in Hormone-Receptor Expression
Another particularly interesting finding involved hormone receptors.
Following exposure to DE-T1, researchers observed increased expression of several genes involved in ovarian signalling, including FSHR, LHR, IGF-1R and CYP19A1.
These receptors and enzymes are involved in the way ovarian cells respond to hormonal signals.
One of the largest changes was observed in IGF-1R expression. In granulosa cells from the poor ovarian response group, the study reported approximately a 25-fold increase in IGF-1R expression at a 2.5% DE-T1 concentration compared with the control.
The results therefore provide a possible biological mechanism through which DE-T1, the researched component contained in SHAWKEA T1, may influence ovarian-cell signalling.
Effects on Oestradiol and Progesterone
Researchers also measured two important reproductive hormones: oestradiol (E2) and progesterone.
As DE-T1 concentrations increased under the experimental conditions, secretion of both oestradiol and progesterone increased.
Together with the observed changes in hormone-receptor and aromatase expression, the researchers proposed that DE-T1 may promote steroid-hormone synthesis through its effects on granulosa-cell proliferation and receptor expression.
What Does This Tell Us About SHAWKEA T1?
This research is particularly relevant to SHAWKEA T1 because DE-T1 is a component contained within SHAWKEA T1.
The study provides laboratory evidence that DE-T1 can interact with human ovarian granulosa cells and influence biological pathways associated with:
- granulosa-cell proliferation and viability
- FSH and LH receptor expression
- IGF-1 receptor expression
- aromatase expression
- oestradiol and progesterone production
These findings help researchers understand the potential cellular mechanisms of DE-T1, providing scientific insight into a component of SHAWKEA T1.
However, the distinction between laboratory research and clinical outcomes is important.
The granulosa cells were obtained from women undergoing IVF, but DE-T1 was studied in vitro after the cells were collected. The study therefore does not establish that taking SHAWKEA T1 increases egg quality, pregnancy rates or IVF success.
Instead, it provides mechanistic evidence showing how the DE-T1 component can interact with human ovarian cells under laboratory conditions.
Continuing Scientific Research into SHAWKEA T1 and DE-T1
This published research contributes to the continuing scientific investigation surrounding SHAWKEA T1 and its DE-T1 component.
By examining granulosa-cell proliferation, hormone receptors and steroid-hormone production, researchers are beginning to understand the biological pathways through which DE-T1 may interact with ovarian cells.
These findings provide a scientific foundation for further investigation into DE-T1, SHAWKEA T1 and reproductive biology, while further clinical research will be important for determining how laboratory findings translate into outcomes in humans.
Scientific Reference
Wang T, Xue B, Shao H, Wang SY, Bai L, Yin CH, et al. Effect of Dandelion Extracts on the Proliferation of Ovarian Granulosa Cells and Expression of Hormone Receptors. Chinese Medical Journal. 2018;131(14):1694–1701. DOI: 10.4103/0366-6999.235864.
This article discusses published scientific research for educational purposes. Laboratory findings involving DE-T1 should not be interpreted as evidence that SHAWKEA T1 can prevent, treat or cure infertility or any other medical condition.
科研聚焦：SHAWKEA T1 中的 DE-T1 如何影响人体卵巢颗粒细胞及激素受体
关于 SHAWKEA T1 的科学研究一直在持续进行。其中，研究人员特别关注 DE-T1——SHAWKEA T1 中所含的蒲公英来源成分，并研究其与卵巢功能相关细胞之间的作用。
一项发表于《中国医学杂志》（Chinese Medical Journal）的研究，专门探讨了 DE-T1 对人体卵巢颗粒细胞的影响，包括颗粒细胞增殖、激素受体表达以及类固醇激素分泌等方面。
这项研究帮助我们进一步了解 SHAWKEA T1 中 DE-T1 成分可能涉及的细胞作用机制。
什么是 DE-T1？
DE-T1 是 SHAWKEA T1 中所含的蒲公英来源成分，也是目前相关科学研究的重要研究对象之一。
在这项研究中，研究人员直接在实验室条件下使用 DE-T1，观察其与人体卵巢颗粒细胞之间的相互作用，从而进一步了解 DE-T1 在细胞层面的生物学作用。
为什么卵巢颗粒细胞如此重要？
卵巢颗粒细胞（Granulosa Cells）是围绕在卵母细胞周围、支持卵泡及卵母细胞发育的重要细胞。
它们参与卵泡内的多种生理过程，并对 促卵泡激素（FSH） 和 促黄体生成素（LH） 等生殖激素产生反应，同时参与 雌二醇（E2）和孕酮 等激素的合成与分泌。
因此，研究颗粒细胞的活性以及激素受体表达，对于理解卵巢的生物学机制具有重要意义。
研究是如何进行的？
研究人员收集了 60 名接受 IVF（体外受精）女性的卵巢颗粒细胞。
参与者分为两组：
- 正常卵巢反应组（NOR）
- 卵巢低反应组（POR）
研究人员将取得的颗粒细胞在体外培养，并加入不同浓度的 DE-T1，随后观察多项与卵巢功能相关的生物学指标。
其中包括：
FSHR —— 促卵泡激素（FSH）受体
LHR —— 黄体生成素（LH）受体
IGF-1R —— 胰岛素样生长因子-1受体，与细胞生长及信号传导有关
CYP19A1（芳香化酶） —— 参与雌激素合成的重要酶
研究人员同时测量了 雌二醇（E2）和孕酮 的分泌情况。
DE-T1 与卵巢颗粒细胞活性
研究结果显示，在实验条件下，随着 DE-T1 浓度增加，颗粒细胞的活力也有所增加。
培养 48 小时后，多个 DE-T1 浓度组的细胞增殖活性明显高于未加入 DE-T1 的对照组。
在来自卵巢低反应（POR）患者的颗粒细胞中，研究人员还观察到，与对照组相比，加入 DE-T1 后，细胞老化、活力下降以及增殖能力下降的过程有所延缓。
这些实验室研究结果表明，SHAWKEA T1 中所含的 DE-T1 成分，在特定实验条件下能够影响人体卵巢颗粒细胞的活性及增殖。
DE-T1 与激素受体表达
这项研究另一个值得关注的结果，是 激素受体表达的变化。
颗粒细胞接触 DE-T1 后，研究人员观察到多种与卵巢信号传导有关的基因表达增加，包括：
FSHR、LHR、IGF-1R 和 CYP19A1。
这些受体和酶参与卵巢细胞对激素信号的识别和反应。
其中一个较为明显的变化出现在 IGF-1R。
在卵巢低反应组的颗粒细胞中，当 DE-T1 浓度为 2.5% 时，研究报告显示 IGF-1R 的表达约为对照组的 25 倍。
这一结果为研究人员进一步探索 DE-T1 如何影响卵巢细胞信号传导 提供了重要的实验依据。
对雌二醇和孕酮的影响
研究人员还检测了两种重要的生殖相关激素：
雌二醇（E2）和孕酮（Progesterone）。
在实验条件下，随着 DE-T1 浓度增加，研究人员观察到 雌二醇和孕酮的分泌量增加。
结合激素受体及芳香化酶表达的变化，研究人员提出，DE-T1 可能通过影响颗粒细胞增殖及相关受体表达，进一步影响类固醇激素的合成。
这项研究对于 SHAWKEA T1 意味着什么？
这项研究与 SHAWKEA T1 密切相关，因为 DE-T1 是 SHAWKEA T1 中所含的蒲公英来源成分。
研究在实验室条件下观察到 DE-T1 与人体卵巢颗粒细胞之间的相互作用，并涉及多个重要的生物学方向，包括：
- 卵巢颗粒细胞的增殖及活力
- FSH 和 LH 受体表达
- IGF-1 受体表达
- 芳香化酶表达
- 雌二醇和孕酮的分泌
这些结果让我们能够从细胞和分子层面，进一步了解 SHAWKEA T1 中 DE-T1 成分的潜在作用机制。
不过，在理解这项研究时，也需要特别注意研究本身的范围。
虽然实验所使用的颗粒细胞来自正在接受 IVF 的女性，但研究人员是在细胞取出后，于 '体外实验室环境（in vitro)' 中加入 DE-T1 进行研究。
因此，这项研究本身并不能证明服用 SHAWKEA T1 可以提高卵子质量、提高怀孕率或提高 IVF 成功率。
它的主要价值在于帮助我们了解 DE-T1 在人体卵巢细胞层面的生物学作用及潜在机制。
SHAWKEA T1 与 DE-T1 的持续科研
这项发表的研究进一步丰富了关于 SHAWKEA T1 及其 DE-T1 成分的科学研究。
通过研究颗粒细胞增殖、激素受体表达以及类固醇激素分泌，研究人员正在逐步了解 DE-T1 与卵巢细胞之间可能存在的生物学作用机制。
这些研究结果也为未来进一步探索 DE-T1、SHAWKEA T1 与生殖生物学提供了科学基础。
未来仍需要更多研究，尤其是人体临床研究，来进一步确定这些实验室发现是否以及如何转化为实际的人体临床结果。
科学文献
Wang T, Xue B, Shao H, Wang SY, Bai L, Yin CH, et al. Effect of Dandelion Extracts on the Proliferation of Ovarian Granulosa Cells and Expression of Hormone Receptors. Chinese Medical Journal. 2018;131(14):1694–1701. DOI: 10.4103/0366-6999.235864.